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Eukaryotic Cell, April 2005, p. 827-831, Vol. 4, No. 4
1535-9778/05/$08.00+0     doi:10.1128/EC.4.4.827-831.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Proteomic Analysis of Phagocytosis in the Enteric Protozoan Parasite Entamoeba histolytica

Mami Okada,1,2,3 Christopher D. Huston,4,{dagger} Barbara J. Mann,4 William A. Petri Jr.,5 Kiyoshi Kita,2 and Tomoyoshi Nozaki1,3*

Department of Parasitology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511,1 Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033,2 Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, 2-20-5 Akebono-cho, Tachikawa, Tokyo 190-0012, Japan,3 Departments of Internal Medicine and Microbiology,4 Department of Medicine, Microbiology, and Pathology, University of Virginia Health System, Charlottesville, Virginia 22908-13405

Received 5 October 2004/ Accepted 21 January 2004

Proteomic analysis of phagosomes isolated from Entamoeba histolytica by liquid chromatography and mass spectrometry identified 85 proteins involved in surface recognition, actin cytoskeleton rearrangement, vesicular trafficking, and degradation. Phagosome localization of representative proteins was verified by immunofluorescence assay. This study should provide a basis for molecular identification and characterization of phagosome biogenesis.

* Corresponding author. Mailing address: Department of Parasitology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan. Phone: 81-27-220-8020. Fax: 81-27-220-8025. E-mail: nozaki{at}med.gunma-u.ac.jp.

{dagger} Present address: Department of Medicine, University of Vermont College of Medicine, Burlington, VT 05401.

Eukaryotic Cell, April 2005, p. 827-831, Vol. 4, No. 4
1535-9778/05/$08.00+0     doi:10.1128/EC.4.4.827-831.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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