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Eukaryotic Cell, April 2005, p. 733-741, Vol. 4, No. 4
1535-9778/05/$08.00+0     doi:10.1128/EC.4.4.733-741.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Effect of the Major Repeat Sequence on Chromosome Loss in Candida albicans

Paul R. Lephart,1 Hiroji Chibana,2 and Paul T. Magee1*

Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota,1 Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan2

Received 28 January 2005/ Accepted 7 February 2005

The major repeat sequence (MRS) is found at least once on all but one chromosome in Candida albicans, but as yet it has no known relation to the phenotype. The MRS affects karyotypic variation by serving as a hot spot for chromosome translocation and by expanding and contracting internal repeats, thereby changing chromosome length. Thus, MRSs on different chromosomes and those on chromosome homologues can differ in size. We proposed that the MRS's unique repeat structure and, more specifically, the size of the MRS could also affect karyotypic variation by altering the frequency of mitotic nondisjunction. Subsequent analysis shows that both natural and artificially induced differences in the size of the chromosome 5 MRS can affect chromosome segregation. Strains with chromosome 5 homologues that differ in the size of the naturally occurring MRSs show a preferential loss of the homologue with the larger MRS on sorbose, indicating that a larger MRS leads to a higher risk of mitotic nondisjunction for that homologue. While deletion of an MRS has no deleterious effect on the deletion chromosome under normal growth conditions and leads to no obvious phenotype, strains that have the MRS deleted from one chromosome 5 homologue preferentially lose the homologue with the MRS remaining. This effect on chromosome segregation is the first demonstration of a phenotype associated with the MRS.

* Corresponding author. Mailing address: Genetics, Cell Biology, and Development, University of Minnesota, 321 Church St. SE, Minneapolis, MN 55455. Phone: (612) 625-4732. E-mail: ptm{at}cbs.umn.edu.

Eukaryotic Cell, April 2005, p. 733-741, Vol. 4, No. 4
1535-9778/05/$08.00+0     doi:10.1128/EC.4.4.733-741.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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