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Eukaryotic Cell, April 2005, p. 685-693, Vol. 4, No. 4
1535-9778/05/$08.00+0     doi:10.1128/EC.4.4.685-693.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Surface Localization of the Yps3p Protein of Histoplasma capsulatum

Megan L. Bohse and Jon P. Woods*

Department of Medical Microbiology and Immunology, University of Wisconsin Medical School, Madison, Wisconsin

Received 4 October 2004/ Accepted 25 January 2005

The YPS3 gene of Histoplasma capsulatum encodes a protein that is both resident in the cell wall and also released into the culture medium. This protein is produced only during the pathogenic yeast phase of infection and is also expressed differently in H. capsulatum strains that differ in virulence. We investigated the cellular localization of Yps3p. We demonstrated that the cell wall fraction of Yps3p was surface localized in restriction fragment length polymorphism class 2 strains. We also established that Yps3p released into the G217B culture supernatant binds to the surface of strains that do not naturally express the protein. This binding was saturable and occurred within 5 min of exposure and occurred similarly with live and heat-killed H. capsulatum. Flow cytometric analysis of H. capsulatum after enzymatic treatments was consistent with Yps3p binding to chitin, a carbohydrate polymer that is a component of fungal cell walls. Polysaccharide binding assays demonstrated that chitin but not cellulose binds to and extracts Yps3p from culture supernatants.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, 420 SMI, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706-1532. Phone: (608) 265-6292. Fax: (608) 265-6132. E-mail: jpwoods{at}wisc.edu.

Eukaryotic Cell, April 2005, p. 685-693, Vol. 4, No. 4
1535-9778/05/$08.00+0     doi:10.1128/EC.4.4.685-693.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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