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Eukaryotic Cell, March 2005, p. 615-624, Vol. 4, No. 3
1535-9778/05/$08.00+0     doi:10.1128/EC.4.3.615-624.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Mutator-Like Element in the Yeast Yarrowia lipolytica Displays Multiple Alternative Splicings{dagger}

Cécile Neuvéglise,1 Fabienne Chalvet,2,{ddagger} Patrick Wincker,3 Claude Gaillardin,1 and Serge Casaregola1*

Microbiologie et Genetique Moleculaire, INRA, CNRS, Institut National Agronomique Paris-Grignon, Thiverval-Grignon,1 Institut de Génétique et Microbiologie, Université Paris Sud, Orsay,2 Génoscope, Centre National de Sequencage, Evry, France3

Received 16 September 2004/ Accepted 4 January 2005

A new type of DNA transposon, Mutyl, has been identified in the sequenced genome of the yeast Yarrowia lipolytica. This transposon is 7,413 bp long and carries two open reading frames (ORFs) which potentially encode proteins of 459 and 1,178 amino acids, respectively. Whereas the first ORF shows no significant homology to previously described proteins, the second ORF shows sequence similarities with various Mutator-like element (MULE)-encoded transposases, including the bacterial transposase signature sequence. Other MULE features shared by Mutyl include a zinc finger motif in the putative transposase, a 22-bp-long imperfect inverted repeat at each end, and a 9- to 10-bp duplication of its target site in the chromosome. Of the five copies of Mutyl present in the genome, one has a deletion of the first 8 bases, and the others are full length with a single base change in one element. The first potential gene of Mutyl, mutB, was shown to be expressed in exponentially growing cells. Its sequence contains a predicted intron with two 5' splice sites, a single branch point, and two 3' splice sites. Its mRNA is alternatively spliced, as judged by reverse transcription-PCR, and generates four mRNAs corresponding to protein-coding sequences of 128, 156, 161, and 190 amino acids. Of the three distinct lineages characterized in Y. lipolytica, strains from the German lineage and the French lineage do not carry Mutyl. A study of the distribution of Mutyl in strains of the French lineage evidenced a recent transposition event. Taken together, these results indicate that Mutyl is still active.

* Corresponding author. Mailing address: Microbiologie et Genetique Moleculaire, CNRS UMR2585, INRA UMR1238, INA-PG, BP 01, 78850 Thiverval-Grignon, France. Phone: 33 1 30 81 52 94. Fax: 33 1 30 81 54 57. E-mail: serge{at}grignon.inra.fr.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

{ddagger} Present address: Mémoire Cellulaire, LGBC, Université de Versailles/Saint-Quentin-en-Yvelines, Versailles, France.

Eukaryotic Cell, March 2005, p. 615-624, Vol. 4, No. 3
1535-9778/05/$08.00+0     doi:10.1128/EC.4.3.615-624.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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