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Eukaryotic Cell, February 2005, p. 484-486, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.484-486.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Rearranged Subtelomeric rRNA Genes in Giardia duodenalis

Jacqueline A. Upcroft,* Mahin Abedinia, and Peter Upcroft

Queensland Institute of Medical Research, The Bancroft Centre, and the Australian Centre for International and Tropical Health and Nutrition, University of Queensland, Brisbane, Queensland, Australia

Received 19 August 2004/ Accepted 25 October 2004

Giardia duodenalis has linear chromosomes capped with typical eukaryotic repeats [(TAGGG)n], subtelomeric rRNA genes, and telomere gene units. The absence of two closely associated NotI sites in the large-subunit rRNA gene was used as an indicator in hybridizations of one- and two-dimensional NotI-cleaved Giardia chromosome separations that some chromosomes carry only rearranged and, by deduction, nonfunctional rRNA genes.

* Corresponding author. Mailing address: Queensland Institute of Medical Research, PO Royal Brisbane Hospital, Queensland 4029, Australia. Phone: 61 7 3362 0369. Fax: 61 7 3362 0105. E-mail: jacquiU{at}qimr.edu.au.

Eukaryotic Cell, February 2005, p. 484-486, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.484-486.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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