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Eukaryotic Cell, February 2005, p. 356-364, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.356-364.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Yarrowia lipolytica Mutants Devoid of Pyruvate Carboxylase Activity Show an Unusual Growth Phenotype{dagger}

Carmen-Lisset Flores* and Carlos Gancedo

Instituto de Investigaciones Biomédicas Alberto Sols, CSIC-Universidad Autónoma de Madrid, Madrid, Spain

Received 26 August 2004/ Accepted 2 December 2004

We have cloned and characterized the gene PYC1, encoding the unique pyruvate carboxylase in the dimorphic yeast Yarrowia lipolytica. The protein putatively encoded by the cDNA has a length of 1,192 amino acids and shows around 70% identity with pyruvate carboxylases from other organisms. The corresponding genomic DNA possesses an intron of 269 bp located 133 bp downstream of the starting ATG. In the branch motif of the intron, the sequence CCCTAAC, not previously found at this place in spliceosomal introns of Y. lipolytica, was uncovered. Disruption of the PYC1 gene from Y. lipolytica did not abolish growth in glucose-ammonium medium, as is the case in other eukaryotic microorganisms. This unusual growth phenotype was due to an incomplete glucose repression of the function of the glyoxylate cycle, as shown by the lack of growth in that medium of double pyc1 icl1 mutants lacking both pyruvate carboxylase and isocitrate lyase activity. These mutants grew when glutamate, aspartate, or Casamino Acids were added to the glucose-ammonium medium. The cDNA from the Y. lipolytica PYC1 gene complemented the growth defect of a Saccharomyces cerevisiae pyc1 pyc2 mutant, but introduction of either the S. cerevisiae PYC1 or PYC2 gene into Y. lipolytica did not result in detectable pyruvate carboxylase activity or in growth on glucose-ammonium of a Y. lipolytica pyc1 icl1 double mutant.

* Corresponding author. Mailing address: Instituto de Investigaciones Biomédicas "Alberto Sols," CSIC-UAM, C/ Arturo Duperier 4, E28029 Madrid, Spain. Phone: 34 91 585 44 31. Fax: 34 91 585 4401. E-mail: clflores{at}iib.uam.es.

{dagger} We dedicate this paper to the memory of Professor Federico Uruburu, microbiologist and friend who devoted much of his activity in the Spanish Type Culture Collection to the service of the microbiological community.

Eukaryotic Cell, February 2005, p. 356-364, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.356-364.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jardon, R., Gancedo, C., Flores, C.-L. (2008). The Gluconeogenic Enzyme Fructose-1,6-Bisphosphatase Is Dispensable for Growth of the Yeast Yarrowia lipolytica in Gluconeogenic Substrates. Eukaryot Cell 7: 1742-1749 [Abstract] [Full Text]  
  • Flores, C.-L., Martinez-Costa, O. H., Sanchez, V., Gancedo, C., Aragon, J. J. (2005). The dimorphic yeast Yarrowia lipolytica possesses an atypical phosphofructokinase: characterization of the enzyme and its encoding gene. Microbiology 151: 1465-1474 [Abstract] [Full Text]  


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