This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lin, C.-Y.
Right arrow Articles by Teng, S.-C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, C.-Y.
Right arrow Articles by Teng, S.-C.

 Previous Article  |  Next Article 

Eukaryotic Cell, February 2005, p. 327-336, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.327-336.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Extrachromosomal Telomeric Circles Contribute to Rad52-, Rad50-, and Polymerase {delta}-Mediated Telomere-Telomere Recombination in Saccharomyces cerevisiae

Chi-Ying Lin,1 Hsih-Hsuan Chang,1 Kou-Juey Wu,2 Shun-Fu Tseng,1 Chuan-Chuan Lin,1 Chao-Po Lin,1,{dagger} and Shu-Chun Teng1*

Department of Microbiology, National Taiwan University College of Medicine,1 Institute of Biochemistry, National Yang-Ming University, Taipei, Taiwan2

Received 28 June 2004/ Accepted 3 November 2004

Telomere maintenance is required for chromosome stability, and telomeres are typically replicated by the telomerase reverse transcriptase. In both tumor and yeast cells that lack telomerase, telomeres are maintained by an alternative recombination mechanism. By using an in vivo inducible Cre-loxP system to generate and trace the fate of marked telomeric DNA-containing rings, the efficiency of telomere-telomere recombination can be determined quantitatively. We show that the telomeric loci are the primary sites at which a marked telomeric ring-containing DNA is observed among wild-type and surviving cells lacking telomerase. Marked telomeric DNAs can be transferred to telomeres and form tandem arrays through Rad52-, Rad50-, and polymerase {delta}-mediated recombination. Moreover, increases of extrachromosomal telomeric and Y' rings were observed in telomerase-deficient cells. These results imply that telomeres can use looped-out telomeric rings to promote telomere-telomere recombination in telomerase-deficient Saccharomyces cerevisiae.

* Corresponding author. Mailing address: Department of Microbiology, National Taiwan University, College of Medicine, No. 1, Sec. 1, Jen-Ai Rd., Taipei 10018, Taiwan. Phone: (886) 2-2312-3456, ext. 8282. Fax: (886) 2-2391-5293. E-mail: scteng{at}ha.mc.ntu.edu.tw.

{dagger} Present address: Department of Pharmacology, University of Medicine and Dentistry of New Jersey—Robert Wood Johnson Medical School, Piscataway, N.J.

Eukaryotic Cell, February 2005, p. 327-336, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.327-336.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Basenko, E. Y., Cesare, A. J., Iyer, S., Griffith, J. D., McEachern, M. J. (2009). Telomeric circles are abundant in the stn1-M1 mutant that maintains its telomeres through recombination. Nucleic Acids Res 0: gkp814v1-gkp814 [Abstract] [Full Text]  
  • LeBel, C., Rosonina, E., Sealey, D. C. F., Pryde, F., Lydall, D., Maringele, L., Harrington, L. A. (2009). Telomere Maintenance and Survival in Saccharomyces cerevisiae in the Absence of Telomerase and RAD52. Genetics 182: 671-684 [Abstract] [Full Text]  
  • Grandin, N., Charbonneau, M. (2009). Telomerase- and Rad52-Independent Immortalization of Budding Yeast by an Inherited-Long-Telomere Pathway of Telomeric Repeat Amplification. Mol. Cell. Biol. 29: 965-985 [Abstract] [Full Text]  
  • Bechard, L. H., Butuner, B. D., Peterson, G. J., McRae, W., Topcu, Z., McEachern, M. J. (2009). Mutant Telomeric Repeats in Yeast Can Disrupt the Negative Regulation of Recombination-Mediated Telomere Maintenance and Create an Alternative Lengthening of Telomeres-Like Phenotype. Mol. Cell. Biol. 29: 626-639 [Abstract] [Full Text]  
  • Cesare, A. J., Groff-Vindman, C., Compton, S. A., McEachern, M. J., Griffith, J. D. (2008). Telomere Loops and Homologous Recombination-Dependent Telomeric Circles in a Kluyveromyces lactis Telomere Mutant Strain. Mol. Cell. Biol. 28: 20-29 [Abstract] [Full Text]  
  • Grudic, A., Jul-Larsen, A., Haring, S. J., Wold, M. S., Lonning, P. E., Bjerkvig, R., Boe, S. O. (2007). Replication protein A prevents accumulation of single-stranded telomeric DNA in cells that use alternative lengthening of telomeres. Nucleic Acids Res 35: 7267-7278 [Abstract] [Full Text]  
  • Pike, B. L., Heierhorst, J. (2007). Mdt1 Facilitates Efficient Repair of Blocked DNA Double-Strand Breaks and Recombinational Maintenance of Telomeres. Mol. Cell. Biol. 27: 6532-6545 [Abstract] [Full Text]  
  • Yu, E. Y., Steinberg-Neifach, O., Dandjinou, A. T., Kang, F., Morrison, A. J., Shen, X., Lue, N. F. (2007). Regulation of Telomere Structure and Functions by Subunits of the INO80 Chromatin Remodeling Complex. Mol. Cell. Biol. 27: 5639-5649 [Abstract] [Full Text]  
  • Iyer, S., Chadha, A. D., McEachern, M. J. (2005). A Mutation in the STN1 Gene Triggers an Alternative Lengthening of Telomere-Like Runaway Recombinational Telomere Elongation and Rapid Deletion in Yeast. Mol. Cell. Biol. 25: 8064-8073 [Abstract] [Full Text]  
  • Cohen, S., Agmon, N., Yacobi, K., Mislovati, M., Segal, D. (2005). Evidence for rolling circle replication of tandem genes in Drosophila. Nucleic Acids Res 33: 4519-4526 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»