This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gildor, T.
Right arrow Articles by Kornitzer, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gildor, T.
Right arrow Articles by Kornitzer, D.

 Previous Article  |  Next Article 

Eukaryotic Cell, February 2005, p. 310-318, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.310-318.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Coevolution of Cyclin Pcl5 and Its Substrate Gcn4

Tsvia Gildor, Revital Shemer, Avigail Atir-Lande, and Daniel Kornitzer*

Department of Molecular Microbiology, B. Rappaport Faculty of Medicine, Technion-IIT, and Rappaport Institute for Research in the Medical Sciences, Haifa, Israel

Received 4 November 2004/ Accepted 29 November 2004

Gcn4, a transcription factor that plays a key role in the response of Saccharomyces cerevisiae to amino acid starvation, is regulated at both the levels of translation and of protein stability. Regulated degradation of Gcn4 depends on its phosphorylation by the cyclin-dependent kinase Pho85, in conjunction with the cyclin Pcl5. The pathogenic yeast Candida albicans contains a functional homolog of Gcn4, which is involved in amino acid metabolism, as well as in the regulation of filamentous growth in response to starvation. Here, we show that C. albicans Gcn4 (CaGcn4) is rapidly degraded and that this degradation depends on a Pho85 cyclin homolog, CaPcl5. The regulatory loop that includes Gcn4 and Pcl5 is conserved in C. albicans: like in S. cerevisiae, CaPcl5 is transcriptionally induced by CaGcn4 and is required for CaGcn4 degradation. However, the proteins have coevolved so that there is no cross-recognition between the proteins from the two species: phosphorylation-dependent degradation of CaGcn4 occurs only in the presence of CaPcl5, and S. cerevisiae Gcn4 (ScGcn4) requires ScPcl5 for its degradation. Phenotypic analysis of the Capcl5 mutant indicates that CaPcl5 also modulates the filamentous response of C. albicans in amino acid-rich media.

* Corresponding author. Mailing address: Department of Molecular Microbiology, B. Rappaport Faculty of Medicine, Technion-IIT, and Rappaport Institute for Research in the Medical Sciences, Haifa, 31096, Israel. Phone: 972-4-829 5258. Fax: 972-4-829 5254 E-mail: danielk{at}techunix.technion.ac.il.

Eukaryotic Cell, February 2005, p. 310-318, Vol. 4, No. 2
1535-9778/05/$08.00+0     doi:10.1128/EC.4.2.310-318.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Trunk, K., Gendron, P., Nantel, A., Lemieux, S., Roemer, T., Raymond, M. (2009). Depletion of the Cullin Cdc53p Induces Morphogenetic Changes in Candida albicans. Eukaryot Cell 8: 756-767 [Abstract] [Full Text]  
  • Streckfuss-Bomeke, K., Schulze, F., Herzog, B., Scholz, E., Braus, G. H. (2009). Degradation of Saccharomyces cerevisiae Transcription Factor Gcn4 Requires a C-Terminal Nuclear Localization Signal in the Cyclin Pcl5. Eukaryot Cell 8: 496-510 [Abstract] [Full Text]  
  • Aviram, S., Simon, E., Gildor, T., Glaser, F., Kornitzer, D. (2008). Autophosphorylation-Induced Degradation of the Pho85 Cyclin Pcl5 Is Essential for Response to Amino Acid Limitation. Mol. Cell. Biol. 28: 6858-6869 [Abstract] [Full Text]  
  • Tournu, H., Tripathi, G., Bertram, G., Macaskill, S., Mavor, A., Walker, L., Odds, F. C., Gow, N. A. R., Brown, A. J. P. (2005). Global Role of the Protein Kinase Gcn2 in the Human Pathogen Candida albicans. Eukaryot Cell 4: 1687-1696 [Abstract] [Full Text]  
  • Atir-Lande, A., Gildor, T., Kornitzer, D. (2005). Role for the SCFCDC4 Ubiquitin Ligase in Candida albicans Morphogenesis. Mol. Biol. Cell 16: 2772-2785 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»