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Eukaryotic Cell, November 2005, p. 1926-1933, Vol. 4, No. 11
1535-9778/05/$08.00+0     doi:10.1128/EC.4.11.1926-1933.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Functional Analysis of the Polyketide Synthase Genes in the Filamentous Fungus Gibberella zeae (Anamorph Fusarium graminearum)

Iffa Gaffoor,1 Daren W. Brown,2 Ron Plattner,2 Robert H. Proctor,2 Weihong Qi,1,{dagger} and Frances Trail1,3*

Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824,1 Mycotoxin Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604,2 Department of Plant Pathology, Michigan State University, East Lansing, Michigan 488243

Received 22 June 2005/ Accepted 7 September 2005

Polyketides are a class of secondary metabolites that exhibit a vast diversity of form and function. In fungi, these compounds are produced by large, multidomain enzymes classified as type I polyketide synthases (PKSs). In this study we identified and functionally disrupted 15 PKS genes from the genome of the filamentous fungus Gibberella zeae. Five of these genes are responsible for producing the mycotoxins zearalenone, aurofusarin, and fusarin C and the black perithecial pigment. A comprehensive expression analysis of the 15 genes revealed diverse expression patterns during grain colonization, plant colonization, sexual development, and mycelial growth. Expression of one of the PKS genes was not detected under any of 18 conditions tested. This is the first study to genetically characterize a complete set of PKS genes from a single organism.

* Corresponding author. Mailing address: Department of Plant Biology, Michigan State University, East Lansing, MI 48824. Phone: (517) 432-2939. Fax: (517) 353-1926. E-mail: trail{at}msu.edu.

{dagger} Present address: National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824.

Eukaryotic Cell, November 2005, p. 1926-1933, Vol. 4, No. 11
1535-9778/05/$08.00+0     doi:10.1128/EC.4.11.1926-1933.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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