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Eukaryotic Cell, November 2005, p. 1882-1891, Vol. 4, No. 11
1535-9778/05/$08.00+0     doi:10.1128/EC.4.11.1882-1891.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Repressors Nrg1 and Nrg2 Regulate a Set of Stress-Responsive Genes in Saccharomyces cerevisiae§

Valmik K. Vyas,1,{dagger} Cristin D. Berkey,2 Takenori Miyao,2,{ddagger} and Marian Carlson1,2*

Integrated Program in Cellular, Molecular and Biophysical Studies,1 Department of Genetics and Development, Columbia University, New York, New York2

Received 27 July 2005/ Accepted 7 September 2005

The yeast Saccharomyces cerevisiae responds to environmental stress by rapidly altering the expression of large sets of genes. We report evidence that the transcriptional repressors Nrg1 and Nrg2 (Nrg1/Nrg2), which were previously implicated in glucose repression, regulate a set of stress-responsive genes. Genome-wide expression analysis identified 150 genes that were upregulated in nrg1{Delta} nrg2{Delta} double mutant cells, relative to wild-type cells, during growth in glucose. We found that many of these genes are regulated by glucose repression. Stress response elements (STREs) and STRE-like elements are overrepresented in the promoters of these genes, and a search of available expression data sets showed that many are regulated in response to a variety of environmental stress signals. In accord with these findings, mutation of NRG1 and NRG2 enhanced the resistance of cells to salt and oxidative stress and decreased tolerance to freezing. We present evidence that Nrg1/Nrg2 not only contribute to repression of target genes in the absence of stress but also limit induction in response to salt stress. We suggest that Nrg1/Nrg2 fine-tune the regulation of a set of stress-responsive genes.

* Corresponding author. Mailing address: 701 W. 168th Street, HSC922, New York, NY 10032. Phone: (212) 305-6314. Fax: (212) 305-1741. E-mail: mbc1{at}columbia.edu.

§ Supplemental material for this article may be found at http://ec.asm.org/.

{ddagger} Present address: S. Matsui & Associates, Tokyo 104-0061, Japan.

Eukaryotic Cell, November 2005, p. 1882-1891, Vol. 4, No. 11
1535-9778/05/$08.00+0     doi:10.1128/EC.4.11.1882-1891.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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