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Eukaryotic Cell, January 2005, p. 90-94, Vol. 4, No. 1
1535-9778/05/$08.00+0     doi:10.1128/EC.4.1.90-94.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The G1 Cyclin Cln3 Regulates Morphogenesis in Candida albicans

Bernardo Chapa y Lazo, Steven Bates,{dagger} and Peter Sudbery*

Department of Molecular Biology and Biotechnology, Sheffield University, Sheffield, United Kingdom

Received 22 September 2004/ Accepted 7 October 2004

In Saccharomyces cerevisiae, the G1 cyclin Cln3 initiates the Start of a mitotic cell cycle in response to size and nutrient inputs. Loss of Cln3 delays but does not prevent Start, due to the eventual Cln3-independent transcription of CLN1 and CLN2. When unbudded cells of the human pathogen Candida albicans were depleted of the G1 cyclin Cln3 they increased in size but did not bud. Thus, unlike S. cerevisiae, Cln3 is essential for budding in C. albicans. However, eventually the large unbudded cells spontaneously produced filamentous forms. The morphology was growth medium dependent; on nutritionally poor medium the polarized outgrowths fulfilled the formal criteria for true hyphae. This state is stable, and continued growth leads to a hyphal mycelium, which invades the agar substratum. Interestingly, it is also required for normal hyphal development, as Cln3-depleted cells develop morphological abnormalities if challenged with hyphal inducing signals such as serum or neutral pH. Taken together, these results show that, in C. albicans, Cln3 has assumed a critical role in coordinating mitotic cell division with differentiation.

* Corresponding author. Mailing address: Sheffield University, Department of Molecular Biology and Biotechnology, Western Bank, Sheffield S10 2TN, United Kingdom. Phone: 44 114 222 6186. Fax: 44 114 272 8697. E-mail: P.Sudbery{at}shef.ac.uk.

{dagger} Present address: University of Aberdeen, Institute of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, United Kingdom.

Eukaryotic Cell, January 2005, p. 90-94, Vol. 4, No. 1
1535-9778/05/$08.00+0     doi:10.1128/EC.4.1.90-94.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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