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Eukaryotic Cell, January 2005, p. 55-62, Vol. 4, No. 1
1535-9778/05/$08.00+0     doi:10.1128/EC.4.1.55-62.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Loss of Meiotic Rereplication Block in Saccharomyces cerevisiae Cells Defective in Cdc28p Regulation

Lyndi M. Rice, Constantine Plakas, and Joseph T. Nickels Jr.*

Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania

Received 6 July 2004/ Accepted 4 November 2004

Cdc28p is the major cyclin-dependent kinase in Saccharomyces cerevisiae. Its activity is required for blocking the reinitiation of DNA replication during mitosis. Here, we show that under conditions where Cdc28p activity is improperly regulated—either through the loss of function of the Schizosaccharomyces pombe wee1 ortholog Swe1p or through the expression of a dominant CDC28 allele, CDC28AF—diploid yeast cells are able to complete several rounds of premeiotic DNA replication within a single meiotic cell cycle. Moreover, a percentage of mutant cells exhibit a "multispore" phenotype, possessing the ability to package more than four spores within a single ascus. These multispored asci contain both even and odd numbers of viable spores. In order for meiotic rereplication and multispore formation to occur, cells must initiate homologous recombination and maintain proper chromosome cohesion during meiosis I. Rad9p- or Rad17p-dependent checkpoint mechanisms are not required for multispore formation and neither are the B-type cyclin Clb6p and the cyclin-dependent kinase inhibitor Sic1p. Finally, we present evidence of a possible role for a Cdc55p-dependent protein phosphatase 2A in initiating meiotic replication.

* Corresponding author. Mailing address: Department of Biochemistry, Drexel University College of Medicine, 245 North 15th St., NCB#11115, MS#497, Philadelphia, PA 19102. Phone: (215) 762-1941. Fax: (215) 762-4452. E-mail: JN27{at}drexel.edu.

Eukaryotic Cell, January 2005, p. 55-62, Vol. 4, No. 1
1535-9778/05/$08.00+0     doi:10.1128/EC.4.1.55-62.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sawarynski, K. E., Najor, N. A., Kepsel, A. C., Brush, G. S. (2009). Sic1-induced DNA rereplication during meiosis. Proc. Natl. Acad. Sci. USA 106: 232-237 [Abstract] [Full Text]  
  • Schindler, K., Winter, E. (2006). Phosphorylation of Ime2 Regulates Meiotic Progression in Saccharomyces cerevisiae. J. Biol. Chem. 281: 18307-18316 [Abstract] [Full Text]  


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