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Next Article 

Eukaryotic Cell, January 2005, p. 17-29, Vol. 4, No. 1
1535-9778/05/$08.00+0     doi:10.1128/EC.4.1.17-29.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Cyclophilin A Is Localized to the Nucleus and Controls Meiosis in Saccharomyces cerevisiae{dagger}

Miguel Arévalo-Rodríguez{ddagger} and Joseph Heitman*

Departments of Molecular Genetics and Microbiology, Pharmacology and Cancer Biology, and Medicine, Howard Hughes Medical Institute, Duke University Medical Center, Durham, North Carolina

Received 17 June 2004/ Accepted 15 October 2004

Cyclophilin A is conserved from yeast to humans and mediates the ability of cyclosporine to perturb signal transduction cascades via inhibition of calcineurin. Cyclophilin A also catalyzes cis-trans peptidyl-prolyl isomerization during protein folding or conformational changes; however, cyclophilin A is not essential in yeast or human cells, and the true biological functions of this highly conserved enzyme have remained enigmatic. In Saccharomyces cerevisiae, cyclophilin A becomes essential in cells compromised for the nuclear prolyl-isomerase Ess1, and cyclophilin A physically interacts with two nuclear histone deacetylase complexes, Sin3-Rpd3 and Set3C, which both control meiosis. Here we show that cyclophilin A is localized to the nucleus in yeast cells and governs the meiotic gene program to promote efficient sporulation. The prolyl-isomerase activity of cyclophilin A is required for this meiotic function. We document that cyclophilin A physically associates with the Set3C histone deacetylase and analyze in detail the structure of this protein-protein complex. Genetic studies support a model in which cyclophilin A controls meiosis via Set3C and an additional target. Our findings reveal a novel nuclear role for cyclophilin A in governing the transcriptional program required for the vegetative to meiotic developmental switch in budding yeast.

* Corresponding author. Mailing address: Department of Molecular Genetics and Microbiology, Box 3546, 322 CARL Bldg., Research Dr., Duke University Medical Center, Durham, NC 27710. Phone: (919) 684-2824. Fax: (919) 684-5458. E-mail: heitm001{at}duke.edu.

{dagger} Supplemental material for this article may be found at http://ec.asm.org/.

{ddagger} Present address: BIOMEDAL Centro Andaluz de Biologiía del Desarrollo, Universidad Pablo de Olavide, 41013 Seville, Spain.

Eukaryotic Cell, January 2005, p. 17-29, Vol. 4, No. 1
1535-9778/05/$08.00+0     doi:10.1128/EC.4.1.17-29.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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  • Colgan, J., Asmal, M., Yu, B., Luban, J. (2005). Cyclophilin A-Deficient Mice Are Resistant to Immunosuppression by Cyclosporine. J. Immunol. 174: 6030-6038 [Abstract] [Full Text]  
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