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Eukaryotic Cell, January 2005, p. 103-110, Vol. 4, No. 1
1535-9778/05/$08.00+0     doi:10.1128/EC.4.1.103-110.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Proposed Function of the Accumulation of Plasma Membrane-Type Ca2+-ATPase mRNA in Resting Cysts of the Ciliate Sterkiella histriomuscorum

Rachel Lescasse, Jeanine Grisvard, Ghislaine Fryd, Anne Fleury-Aubusson, and Anne Baroin-Tourancheau*

Laboratoire de Biologie Cellulaire 4, Université Paris-Sud, Orsay, France

Received 25 June 2004/ Accepted 18 October 2004

From an mRNA differential-display analysis of the encystment-excystment cycle of the ciliate Sterkiella histriomuscorum, we have isolated an expressed sequence tag encoding a plasma membrane-type Ca2+-ATPase (PMCA). PMCAs are located either in the plasma membranes or in the membranes of intracellular organelles, and their function is to pump calcium either out of the cell or into the intracellular calcium stores, respectively. The S. histriomuscorum macronuclear PMCA gene (ShPMCA) and its corresponding cDNA were cloned; it is the first member of the Ca2+-ATPase family identified in Sterkiella. The predicted protein of 1,065 amino acids exhibits 37% identity with PMCAs of diverse organisms. A phylogenetic analysis showed its relatedness to homologs of two alveolates: the ciliate Paramecium tetraurelia and the apicomplexan Toxoplasma gondii. Overexpression of the protein ShPMCA failed to rescue the wild-type phenotype of three Ca2+-ATPase-defective mutant strains of Saccharomyces cerevisiae; this failure contrasts with the reported ability of the PMCAs of parasites to complement defects in yeast. ShPMCA mRNA is markedly accumulated during encystment and in resting cysts, suggesting a function during excystment. To address the possibility of a signaling role for calcium at excystment, the capacity of calcium to induce excystment was examined.

* Corresponding author. Mailing address: Laboratoire de Biologie Cellulaire 4, CNRS (UMR 8080), Btiment 444, Université Paris-Sud, 91405 Orsay Cedex, France. Phone: 33-1 69 15 64 84. Fax: 33-1 69 15 68 03. E-mail: anne.baroin{at}ibaic.u-psud.fr.

Eukaryotic Cell, January 2005, p. 103-110, Vol. 4, No. 1
1535-9778/05/$08.00+0     doi:10.1128/EC.4.1.103-110.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.





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