This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jin, H.
Right arrow Articles by Grote, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jin, H.
Right arrow Articles by Grote, E.

 Previous Article

Eukaryotic Cell, December 2004, p. 1664-1673, Vol. 3, No. 6
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.6.1664-1673.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Prm1 Prevents Contact-Dependent Lysis of Yeast Mating Pairs{dagger}

Hui Jin, Candice Carlile, Scott Nolan, and Eric Grote*

Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland

Received 13 July 2004/ Accepted 16 September 2004

Membrane fusion requires localized destabilization of two phospholipid bilayers, but unrestrained membrane destabilization could result in lysis. prm1 mutant yeast cells have a defect at the plasma membrane fusion stage of mating that typically results in the accumulation of prezygotes that have fingers of membrane-bound cytoplasm projecting from one cell of each pair into its mating partner in the direction of the osmotic gradient between the cells. However, some prm1 mating pairs fuse successfully whereas the two cells in other prm1 mating pairs simultaneously lyse. Lysis only occurs if both mating partners are prm1 mutants. Osmotic stabilization does not protect prm1 mating pairs from lysis, indicating that lysis is not caused by a cell wall defect. prm1 mating pairs without functional mitochondria still lyse, ruling out programmed cell death. No excess lysis was found after pheromone treatment of haploid prm1 cells, and lysis did not occur in mating pairs when prm1 was combined with the fus1 and fus2 mutations to block cell wall remodeling. Furthermore, short (<1 µm) cytoplasmic microfingers indicating the completion of cell wall remodeling appeared immediately before lysis. In combination, these results demonstrate that plasma membrane contact is a prerequisite for lysis. Cytoplasmic microfingers are unlikely to cause lysis since most prm1 mating pairs with microfingers do not lyse, and microfingers were also detected before fusion in some wild-type mating pairs. The lysis of prm1 mutant mating pairs suggests that the Prm1 protein stabilizes the membrane fusion event of yeast mating.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, 615 N. Wolfe St., Baltimore, MD 21205. Phone: (443) 287-4989. Fax: (410) 955-2926. E-mail: egrote{at}jhsph.edu.

{dagger} Supplemental material for this article can be found at http://ec.asm.org.

Eukaryotic Cell, December 2004, p. 1664-1673, Vol. 3, No. 6
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.6.1664-1673.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Fleissner, A., Diamond, S., Glass, N. L. (2009). The Saccharomyces cerevisiae PRM1 Homolog in Neurospora crassa Is Involved in Vegetative and Sexual Cell Fusion Events but Also Has Postfertilization Functions. Genetics 181: 497-510 [Abstract] [Full Text]  
  • Engel, A., Walter, P. (2008). Membrane lysis during biological membrane fusion: collateral damage by misregulated fusion machines. JCB 183: 181-186 [Abstract] [Full Text]  
  • Paterson, J. M., Ydenberg, C. A., Rose, M. D. (2008). Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating. JCB 181: 697-709 [Abstract] [Full Text]  
  • Jin, H., McCaffery, J. M., Grote, E. (2008). Ergosterol promotes pheromone signaling and plasma membrane fusion in mating yeast. JCB 180: 813-826 [Abstract] [Full Text]  
  • Starai, V. J., Jun, Y., Wickner, W. (2007). From the Cover: Feature Article: Excess vacuolar SNAREs drive lysis and Rab bypass fusion. Proc. Natl. Acad. Sci. USA 104: 13551-13558 [Abstract] [Full Text]  
  • Shi, C., Kaminskyj, S., Caldwell, S., Loewen, M. C. (2007). A role for a complex between activated G protein-coupled receptors in yeast cellular mating. Proc. Natl. Acad. Sci. USA 104: 5395-5400 [Abstract] [Full Text]  
  • Aguilar, P. S., Engel, A., Walter, P. (2007). The Plasma Membrane Proteins Prm1 and Fig1 Ascertain Fidelity of Membrane Fusion during Yeast Mating. Mol. Biol. Cell 18: 547-556 [Abstract] [Full Text]  
  • Heiman, M. G., Engel, A., Walter, P. (2007). The Golgi-resident protease Kex2 acts in conjunction with Prm1 to facilitate cell fusion during yeast mating. JCB 176: 209-222 [Abstract] [Full Text]  
  • Zhang, N.-N., Dudgeon, D. D., Paliwal, S., Levchenko, A., Grote, E., Cunningham, K. W. (2006). Multiple Signaling Pathways Regulate Yeast Cell Death during the Response to Mating Pheromones. Mol. Biol. Cell 17: 3409-3422 [Abstract] [Full Text]  
  • Barale, S., McCusker, D., Arkowitz, R. A. (2006). Cdc42p GDP/GTP Cycling Is Necessary for Efficient Cell Fusion during Yeast Mating. Mol. Biol. Cell 17: 2824-2838 [Abstract] [Full Text]  
  • Nolan, S., Cowan, A. E., Koppel, D. E., Jin, H., Grote, E. (2006). FUS1 Regulates the Opening and Expansion of Fusion Pores between Mating Yeast. Mol. Biol. Cell 17: 2439-2450 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»