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Eukaryotic Cell, December 2004, p. 1609-1618, Vol. 3, No. 6
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.6.1609-1618.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Snf7p, a Component of the ESCRT-III Protein Complex, Is an Upstream Member of the RIM101 Pathway in Candida albicans

Amy L. Kullas, Mingchun Li, and Dana A. Davis*

Department of Microbiology, University of Minnesota, Minneapolis, Minnesota

Received 13 August 2004/ Accepted 8 October 2004

The success of Candida albicans as an opportunistic pathogen is based in part on its ability to adapt to diverse environments. The RIM101 pathway governs adaptation to neutral-alkaline environments and is required for virulence. Analysis of a genomic two-hybrid study conducted with Saccharomyces cerevisiae revealed that components involved in multivesicular bodies (MVB) transport may interact with RIM101 pathway members. Thus, we hypothesized that these proteins may function in the RIM101 pathway in C. albicans. We identified C. albicans homologs to S. cerevisiae Snf7p, Vps4p, and Bro1p and generated mutants in the cognate gene. We found that snf7{Delta}/{Delta} mutants, but not vps4{Delta}/{Delta} nor bro1{Delta}/{Delta} mutants, had phenotypes similar to, but more severe than, those of RIM101 pathway mutants. We found that the constitutively active RIM101-405 allele partially rescued snf7{Delta}/{Delta} mutant phenotypes. The vps4{Delta}/{Delta} mutant had subtle phenotypes, but these were not rescued by the RIM101-405 allele. Further, we found that the snf7{Delta}/{Delta}, vps4{Delta}/{Delta}, and bro1{Delta}/{Delta} mutants did not efficiently localize the vital dye FM4-64 to the vacuole and that it was often accumulated in an MVB-like compartment. This phenotype was not rescued by RIM101-405 or observed in RIM101 pathway mutants. These results suggest that Snf7p may serve two functions in the cell: one as a RIM101 pathway member and one for MVB transport to the vacuole.

* Corresponding author. Mailing address: Department of Microbiology, University of Minnesota, 1360 Mayo Building MMC196, 420 Delaware St., Minneapolis, MN 55455. Phone: (612) 624-1912. Fax: (612) 626-0623. E-mail: dadavis{at}umn.edu.

Eukaryotic Cell, December 2004, p. 1609-1618, Vol. 3, No. 6
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.6.1609-1618.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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