Eukaryotic Cell
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Eukaryotic Cell, October 2004, p. 1359-1362, Vol. 3, No. 5
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.5.1359-1362.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rapid Production of Gene Replacement Constructs and Generation of a Green Fluorescent Protein-Tagged Centromeric Marker in Aspergillus nidulans

Lin Yang, Leena Ukil, Aysha Osmani, Francis Nahm, Jonathan Davies, Colin P. C. De Souza, Xiaowei Dou, Ariadna Perez-Balaguer, and Stephen A. Osmani*

Department of Molecular Genetics, The Ohio State University, Columbus, Ohio

Received 26 May 2004/ Accepted 12 July 2004

A method to rapidly generate gene replacement constructs by fusion PCR is described for Aspergillus nidulans. The utility of the approach is demonstrated by green fluorescent protein (GFP) tagging of A. nidulans ndc80 to visualize centromeres through the cell cycle. The methodology makes possible large-scale GFP tagging, promoter swapping, and deletion analysis of A. nidulans.

* Corresponding author. Mailing address: Department of Molecular Genetics, The Ohio State University, 804 Riffe Building, 496 West 12th Ave., Columbus, OH 43210. Phone: (614) 247-6791. Fax: (614) 247-6845. E-mail: Osmani.2{at}osu.edu.

Eukaryotic Cell, October 2004, p. 1359-1362, Vol. 3, No. 5
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.5.1359-1362.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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