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Eukaryotic Cell, October 2004, p. 1272-1286, Vol. 3, No. 5
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.5.1272-1286.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Functional Characterization of Myosin I Tail Regions in Candida albicans{dagger}

Ursula Oberholzer,1* Tatiana L. Iouk,1,2 David Y. Thomas,2 and Malcolm Whiteway1,3

Genetics Group, Biotechnology Research Institute, National Research Council of Canada,1 Departments of Biology,3 Biochemistry, McGill University, Montreal, Quebec, Canada2

Received 21 May 2004/ Accepted 6 August 2004

The molecular motor myosin I is required for hyphal growth in the pathogenic yeast Candida albicans. Specific myosin I functions were investigated by a deletion analysis of five neck and tail regions. Hyphal formation requires both the TH1 region and the IQ motifs. The TH2 region is important for optimal hyphal growth. All of the regions, except for the SH3 and acidic (A) regions that were examined individually, were required for the localization of myosin I at the hyphal tip. Similarly, all of the domains were required for the association of myosin I with pelletable actin-bound complexes. Moreover, the hyphal tip localization of cortical actin patches, identified by both rhodamine-phalloidin staining and Arp3-green fluorescent protein signals, was dependent on myosin I. Double deletion of the A and SH3 domains depolarized the distribution of the cortical actin patches without affecting the ability of the mutant to form hyphae, suggesting that myosin I has distinct functions in these processes. Among the six myosin I tail domain mutants, the ability to form hyphae was strictly correlated with endocytosis. We propose that the uptake of cell wall remodeling enzymes and excess plasma membrane is critical for hyphal formation.

* Corresponding author. Mailing address: Genetics Group, Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec H3A 2B2, Canada. Phone: (514) 496-6358. Fax: (514) 496-6213. E-mail: ursula.oberholzer{at}nrc.ca.

{dagger} This is NRC publication number 46 222.

Eukaryotic Cell, October 2004, p. 1272-1286, Vol. 3, No. 5
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.5.1272-1286.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Douglas, L. M., Martin, S. W., Konopka, J. B. (2009). BAR Domain Proteins Rvs161 and Rvs167 Contribute to Candida albicans Endocytosis, Morphogenesis, and Virulence. Infect. Immun. 77: 4150-4160 [Abstract] [Full Text]  
  • Steinberg, G. (2007). Hyphal Growth: a Tale of Motors, Lipids, and the Spitzenkorper. Eukaryot Cell 6: 351-360 [Full Text]  
  • Oberholzer, U., Nantel, A., Berman, J., Whiteway, M. (2006). Transcript Profiles of Candida albicans Cortical Actin Patch Mutants Reflect Their Cellular Defects: Contribution of the Hog1p and Mkc1p Signaling Pathways.. Eukaryot Cell 5: 1252-1265 [Abstract] [Full Text]  


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