This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sadanandom, A.
Right arrow Articles by Shirasu, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sadanandom, A.
Right arrow Articles by Shirasu, K.

 Previous Article  |  Next Article 

Eukaryotic Cell, August 2004, p. 984-991, Vol. 3, No. 4
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.4.984-991.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

CHPA, a Cysteine- and Histidine-Rich-Domain-Containing Protein, Contributes to Maintenance of the Diploid State in Aspergillus nidulans

Ari Sadanandom,1,2 Kim Findlay,3 John H. Doonan,3 Paul Schulze-Lefert,1,{dagger} and Ken Shirasu1*

The Sainsbury Laboratory,1 Department of Cell and Developmental Biology, John Innes Centre,3 Norwich NR4 7UH, and Institute of Biomedical and Lifesciences, University of Glasgow, Glasgow G12 8QQ,United Kingdom2

Received 10 November 2003/ Accepted 7 May 2004

The alternation of eukaryotic life cycles between haploid and diploid phases is crucial for maintaining genetic diversity. In some organisms, the growth and development of haploid and diploid phases are nearly identical, and one might suppose that all genes required for one phase are likely to be critical for the other phase. Here, we show that targeted disruption of the chpA (cysteine- and histidine-rich-domain- [CHORD]-containing protein A) gene in haploid Aspergillus nidulans strains gives rise to chpA knockout haploids and heterozygous diploids but no chpA knockout diploids. A. nidulans chpA heterozygous diploids showed impaired conidiophore development and reduced conidiation. Deletion of chpA from diploid A. nidulans resulted in genome instability and reversion to a haploid state. Thus, our data suggest a vital role for chpA in maintenance of the diploid phase in A. nidulans. Furthermore, the human chpA homolog, Chp-1, was able to complement haploinsufficiency in A. nidulans chpA heterozygotes, suggesting that the function of CHORD-containing proteins is highly conserved in eukaryotes.

* Corresponding author. Mailing address: The Sainsbury Laboratory, John Innes Centre, Colney Ln., Norwich NR4 7UH, United Kingdom. Phone: 44-1603450425. Fax: 44-1603450011. E-mail: ken.shirasu{at}sainsbury-laboratory.ac.uk.

{dagger} Present address: Max-Planck-Institut für Zuechtungsforschung, D-50829 Cologne, Germany.

Eukaryotic Cell, August 2004, p. 984-991, Vol. 3, No. 4
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.4.984-991.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Boter, M., Amigues, B., Peart, J., Breuer, C., Kadota, Y., Casais, C., Moore, G., Kleanthous, C., Ochsenbein, F., Shirasu, K., Guerois, R. (2007). Structural and Functional Analysis of SGT1 Reveals That Its Interaction with HSP90 Is Required for the Accumulation of Rx, an R Protein Involved in Plant Immunity. Plant Cell 19: 3791-3804 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»