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Eukaryotic Cell, August 2004, p. 910-918, Vol. 3, No. 4
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.4.910-918.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

RSC1 and RSC2 Are Required for Expression of Mid-Late Sporulation-Specific Genes in Saccharomyces cerevisiae

David Bungard, Michelle Reed, and Edward Winter*

Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, Pennsylvania 19107

Received 2 June 2004/ Accepted 6 June 2004

Rsc1 and Rsc2 are alternative bromodomain-containing subunits of the ATP-dependent RSC chromatin remodeling complex in Saccharomyces cerevisiae. Smk1 is a sporulation-specific mitogen-activated protein kinase homolog that is required for the postmeiotic events of spore formation. In this study we show that RSC1 and RSC2 are haploinsufficient for spore formation in a smk1 hypomorph. Moreover, diploids lacking Rsc1 or Rsc2 show a subset of smk1-like phenotypes. High-copy-number RSC1 plasmids do not suppress rsc2-{Delta}/rsc2-{Delta} sporulation defects, and high-copy-number RSC2 plasmids do not suppress rsc1-{Delta}/rsc1-{Delta} sporulation defects. Mid-late sporulation-specific genes, which are normally expressed while key steps in spore assembly occur and which include genes that are required for spore wall formation, are not expressed in cells lacking Rsc1 or Rsc2. We speculate that the combined action of Rsc1 and Rsc2 at mid-late promoters is specifically required for the proper expression of this uniquely timed set of genes. Our data suggest that Smk1 and Rsc1/2 define parallel pathways that converge to provide signaling information and the expression of gene products, respectively, that are required for spore morphogenesis.

* Corresponding author. Mailing address: Thomas Jefferson University, 233 S. 10th St., Rm. 228, Philadelphia, PA 19107. Phone: (215) 503-4139. Fax: (215) 923-9162. E-mail: winter{at}lac.jci.tju.edu.

Eukaryotic Cell, August 2004, p. 910-918, Vol. 3, No. 4
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.4.910-918.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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Copyright © 2004 by the American Society for Microbiology.