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Eukaryotic Cell, August 2004, p. 1049-1061, Vol. 3, No. 4
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.4.1049-1061.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Exchange Factor Cdc24 Is Required for Cell Fusion during Yeast Mating

Sophie Barale,1 Derek McCusker,2 and Robert A. Arkowitz1*

Institute of Signaling, Developmental Biology, and Cancer, CNRS UMR 6543, Faculté des Sciences, Université de Nice, 06108 Nice Cedex 2, France,1 Department of Biology, Sinsheimer Laboratories, University of California at Santa Cruz, Santa Cruz, California 950642

Received 28 April 2004/ Accepted 4 May 2004

During Saccharomyces cerevisiae mating, chemotropic growth and cell fusion are critical for zygote formation. Cdc24p, the guanine nucleotide exchange factor for the Cdc42 G protein, is necessary for oriented growth along a pheromone gradient during mating. To understand the functions of this critical Cdc42p activator, we identified additional cdc24 mating mutants. Two mating-specific mutants, the cdc24-m5 and cdc24-m6 mutants, each were isolated with a mutated residue in the conserved catalytic domain. The cdc24-m6 mutant responds normally to pheromone and orients its growth towards a mating partner yet accumulates prezygotes during mating. cdc24-m6 prezygotes have two apposed intact cell walls and do not correctly localize proteins required for cell fusion, despite normal exocytosis. Our results indicate that the exchange factor Cdc24p is necessary for maintaining or restricting specific proteins required for cell fusion to the cell contact region during mating.

* Corresponding author. Mailing address: Institute of Signaling, Developmental Biology, and Cancer, CNRS UMR 6543, Faculté des Sciences-Parc Valrose, Université de Nice, 06108 Nice Cedex 2, France. Phone: 33 (0)4 92 07 6425. Fax: 33 (0)4 92 07 6466. E-mail: arkowitz{at}unice.fr.

Eukaryotic Cell, August 2004, p. 1049-1061, Vol. 3, No. 4
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.4.1049-1061.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Arkowitz, R. A. (2009). Chemical Gradients and Chemotropism in Yeast. Cold Spring Harb. Perspect. Biol. 1: a001958-a001958 [Abstract] [Full Text]  
  • Park, H.-O., Bi, E. (2007). Central Roles of Small GTPases in the Development of Cell Polarity in Yeast and Beyond. Microbiol. Mol. Biol. Rev. 71: 48-96 [Abstract] [Full Text]  
  • Barale, S., McCusker, D., Arkowitz, R. A. (2006). Cdc42p GDP/GTP Cycling Is Necessary for Efficient Cell Fusion during Yeast Mating. Mol. Biol. Cell 17: 2824-2838 [Abstract] [Full Text]  
  • Bassilana, M., Arkowitz, R. A. (2006). Rac1 and Cdc42 Have Different Roles in Candida albicans Development. Eukaryot Cell 5: 321-329 [Abstract] [Full Text]  
  • Bassilana, M., Hopkins, J., Arkowitz, R. A. (2005). Regulation of the Cdc42/Cdc24 GTPase Module during Candida albicans Hyphal Growth. Eukaryot Cell 4: 588-603 [Abstract] [Full Text]  


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