This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rischitor, P. E.
Right arrow Articles by Fischer, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rischitor, P. E.
Right arrow Articles by Fischer, R.

 Previous Article  |  Next Article 

Eukaryotic Cell, June 2004, p. 632-645, Vol. 3, No. 3
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.3.632-645.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Kip3-Like Kinesin KipB Moves along Microtubules and Determines Spindle Position during Synchronized Mitoses in Aspergillus nidulans Hyphae{dagger}

Patricia E. Rischitor, Sven Konzack, and Reinhard Fischer*

Department of Microbiology, University of Marburg, and Max-Planck-Institute for Terrestrial Microbiology, D-35043 Marburg, Germany

Received 5 November 2003/ Accepted 2 March 2004

Kinesins are motor proteins which are classified into 11 different families. We identified 11 kinesin-like proteins in the genome of the filamentous fungus Aspergillus nidulans. Relatedness analyses based on the motor domains grouped them into nine families. In this paper, we characterize KipB as a member of the Kip3 family of microtubule depolymerases. The closest homologues of KipB are Saccharomyces cerevisiae Kip3 and Schizosaccharomyces pombe Klp5 and Klp6, but sequence similarities outside the motor domain are very low. A disruption of kipB demonstrated that it is not essential for vegetative growth. kipB mutant strains were resistant to high concentrations of the microtubule-destabilizing drug benomyl, suggesting that KipB destabilizes microtubules. kipB mutations caused a failure of spindle positioning in the cell, a delay in mitotic progression, an increased number of bent mitotic spindles, and a decrease in the depolymerization of cytoplasmic microtubules during interphase and mitosis. Meiosis and ascospore formation were not affected. Disruption of the kipB gene was synthetically lethal in combination with the temperature-sensitive mitotic kinesin motor mutation bimC4, suggesting an important but redundant role of KipB in mitosis. KipB localized to cytoplasmic, astral, and mitotic microtubules in a discontinuous pattern, and spots of green fluorescent protein moved along microtubules toward the plus ends.

* Corresponding author. Mailing address: Department of Microbiology, University of Marburg, Karl-von-Frisch Str., D-35043 Marburg, Germany. Phone: 49-6421-178-330. Fax: 49-6421-178-309. E-mail: FischerR{at}staff.uni-marburg.de.

{dagger} Supplemental material for this article may be found at http://ec.asm.org.

Eukaryotic Cell, June 2004, p. 632-645, Vol. 3, No. 3
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.3.632-645.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Grissom, P. M., Fiedler, T., Grishchuk, E. L., Nicastro, D., West, R. R., Richard McIntosh, J. (2009). Kinesin-8 from Fission Yeast: A Heterodimeric, Plus-End-directed Motor that Can Couple Microtubule Depolymerization to Cargo Movement. Mol. Biol. Cell 20: 963-972 [Abstract] [Full Text]  
  • Zekert, N., Fischer, R. (2009). The Aspergillus nidulans Kinesin-3 UncA Motor Moves Vesicles along a Subpopulation of Microtubules. Mol. Biol. Cell 20: 673-684 [Abstract] [Full Text]  
  • Finley, K. R., Bouchonville, K. J., Quick, A., Berman, J. (2008). Dynein-dependent nuclear dynamics affect morphogenesis in Candida albicans by means of the Bub2p spindle checkpoint. J. Cell Sci. 121: 466-476 [Abstract] [Full Text]  
  • Enke, C., Zekert, N., Veith, D., Schaaf, C., Konzack, S., Fischer, R. (2007). Aspergillus nidulans Dis1/XMAP215 Protein AlpA Localizes to Spindle Pole Bodies and Microtubule Plus Ends and Contributes to Growth Directionality. Eukaryot Cell 6: 555-562 [Abstract] [Full Text]  
  • Veith, D., Scherr, N., Efimov, V. P., Fischer, R. (2005). Role of the spindle-pole-body protein ApsB and the cortex protein ApsA in microtubule organization and nuclear migration in Aspergillus nidulans. J. Cell Sci. 118: 3705-3716 [Abstract] [Full Text]  
  • Li, S., Oakley, C. E., Chen, G., Han, X., Oakley, B. R., Xiang, X. (2005). Cytoplasmic Dynein's Mitotic Spindle Pole Localization Requires a Functional Anaphase-promoting Complex, {gamma}-Tubulin, and NUDF/LIS1 in Aspergillus nidulans. Mol. Biol. Cell 16: 3591-3605 [Abstract] [Full Text]  
  • Zhu, C., Zhao, J., Bibikova, M., Leverson, J. D., Bossy-Wetzel, E., Fan, J.-B., Abraham, R. T., Jiang, W. (2005). Functional Analysis of Human Microtubule-based Motor Proteins, the Kinesins and Dyneins, in Mitosis/Cytokinesis Using RNA Interference. Mol. Biol. Cell 16: 3187-3199 [Abstract] [Full Text]  
  • Sampson, K., Heath, I. B. (2005). The dynamic behaviour of microtubules and their contributions to hyphal tip growth in Aspergillus nidulans. Microbiology 151: 1543-1555 [Abstract] [Full Text]  
  • Konzack, S., Rischitor, P. E., Enke, C., Fischer, R. (2005). The Role of the Kinesin Motor KipA in Microtubule Organization and Polarized Growth of Aspergillus nidulans. Mol. Biol. Cell 16: 497-506 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»