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Eukaryotic Cell, June 2004, p. 589-597, Vol. 3, No. 3
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.3.589-597.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Deletion of the Succinate Dehydrogenase Gene KlSDH1 in Kluyveromyces lactis Does Not Lead to Respiratory Deficiency

Michele Saliola,1* Paola Chiara Bartoccioni,1,{dagger} Ilaria De Maria,1 Tiziana Lodi,2 and Claudio Falcone1

Dipartimento di Biologia Cellulare e dello Sviluppo, Università di Roma "La Sapienza," 00185 Rome,1 Dipartimento di Genetica, Antropologia e Evoluzione, Università degli Studi di Parma, 43100 Parma, Italy2

Received 17 November 2003/ Accepted 29 March 2004

We have isolated a Kluyveromyces lactis mutant unable to grow on all respiratory carbon sources with the exception of lactate. Functional complementation of this mutant led to the isolation of KlSDH1, the gene encoding the flavoprotein subunit of the succinate dehydrogenase (SDH) complex, which is essential for the aerobic utilization of carbon sources. Despite the high sequence conservation of the SDH genes in Saccharomyces cerevisiae and K. lactis, they do not have the same relevance in the metabolism of the two yeasts. In fact, unlike SDH1, KlSDH1 was highly expressed under both fermentative and nonfermentative conditions. In addition to this, but in contrast with S. cerevisiae, K. lactis strains lacking KlSDH1 were still able to grow in the presence of lactate. In these mutants, oxygen consumption was one-eighth that of the wild type in the presence of lactate and was normal with glucose and ethanol, indicating that the respiratory chain was fully functional. Northern analysis suggested that alternative pathway(s), which involves pyruvate decarboxylase and the glyoxylate cycle, could overcome the absence of SDH and allow (i) lactate utilization and (ii) the accumulation of succinate instead of ethanol during growth on glucose.

* Corresponding author. Mailing address: Department of Developmental and Cell Biology, University of Rome "La Sapienza," Piazzale Aldo Moro 5, 00185 Rome, Italy. Phone: 39 06 49912544. Fax: 39 06 49912351. E-mail: michele.saliola{at}uniroma1.it.

{dagger} Present address: Parc Cientific di Barcelona, 08028 Barcelona, Spain.

Eukaryotic Cell, June 2004, p. 589-597, Vol. 3, No. 3
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.3.589-597.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Saliola, M., Scappucci, G., De Maria, I., Lodi, T., Mancini, P., Falcone, C. (2007). Deletion of the Glucose-6-Phosphate Dehydrogenase Gene KlZWF1 Affects both Fermentative and Respiratory Metabolism in Kluyveromyces lactis. Eukaryot Cell 6: 19-27 [Abstract] [Full Text]  


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