Eukaryotic Cell
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Eukaryotic Cell, April 2004, p. 553-556, Vol. 3, No. 2
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.2.553-556.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Dual-Color Imaging of Nuclear Division and Mitotic Spindle Elongation in Live Cells of Aspergillus nidulans

Wenqi Su,1,2 Shihe Li,1 Berl R. Oakley,3 and Xin Xiang1*

Department of Biochemistry and Molecular Biology, Uniformed Services University of the Health Sciences,1 Walter Johnson High School, Bethesda, Maryland 20814,2 Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 432103

Received 10 December 2003/ Accepted 16 December 2003

We have developed a dual-color imaging system based on cyan fluorescent protein-labeled histone H2A and green fluorescent protein-labeled alpha tubulin to visualize DNA and spindles simultaneously in the same living cell of Aspergillus nidulans. This system allows new details of mitosis and nuclear movement to be revealed.

* Corresponding author. Mailing address: Uniformed Services University of the Health Sciences, Department of Biochemistry and Molecular Biology, 4301 Jones Bridge Rd., Bethesda, MD 20814. Phone: (301) 295-0000. Fax: (301) 295-3512. E-mail: xxiang{at}usuhs.mil.

Eukaryotic Cell, April 2004, p. 553-556, Vol. 3, No. 2
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.2.553-556.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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