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Eukaryotic Cell, April 2004, p. 331-338, Vol. 3, No. 2
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.2.331-338.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Translational Accuracy during Exponential, Postdiauxic, and Stationary Growth Phases in Saccharomyces cerevisiae

Guillaume Stahl,1,2* Samia N. Ben Salem,1,2 Lifeng Chen,1,2 Bing Zhao,1,2 and Philip J. Farabaugh1,2

Department of Biological Sciences,1 Program in Molecular and Cell Biology, University of Maryland Baltimore County, Baltimore, Maryland 212502

Received 11 September 2003/ Accepted 23 January 2004

When the yeast Saccharomyces cerevisiae shifts from rapid growth on glucose to slow growth on ethanol, it undergoes profound changes in cellular metabolism, including the destruction of most of the translational machinery. We have examined the effect of this metabolic change, termed the diauxic shift, on the frequency of translational errors. Recoding sites are mRNA sequences that increase the frequency of translational errors, providing a convenient reporter of translational accuracy. We found that the diauxic shift causes no overall change in translational accuracy but does cause a strong reduction in the frequency of one type of programmed error: Ty +1 frameshifting. Genetic data suggest that this effect may be due to changes in the relative amounts of tRNA participating in translation elongation. We discuss possible implications for expression strategies that use recoding.

* Corresponding author. Present address: LMGM-CNRS, 118 Rt. de Narbonne, 31062 Toulouse, France. Phone: (33) 5 61 33 59 87. Fax: (33) 5 61 33 58 86. E-mail: stahl{at}infobiogen.fr.

Eukaryotic Cell, April 2004, p. 331-338, Vol. 3, No. 2
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.2.331-338.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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