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Eukaryotic Cell, February 2004, p. 241-244, Vol. 3, No. 1
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.1.241-244.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

SmD1 Is Required for Spliced Leader RNA Biogenesis

Gusti M. Zeiner,^1, Silvie Foldynová,^2, Nancy R. Sturm,¹ Julius Luke,² and David A. Campbell^1*

Department of Microbiology, Immunology & Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095-1489,¹ Institute of Parasitology, Czech Academy of Sciences, and Faculty of Biology, University of South Bohemia, Ceské Budejovice, Czech Republic²

Received 22 August 2003/ Accepted 10 December 2003

The Sm-binding site of the kinetoplastid spliced leader RNA has been implicated in accurate spliced leader RNA maturation and trans-splicing competence. In Trypanosoma brucei, RNA interference-mediated knockdown of SmD1 caused defects in spliced leader RNA maturation, displaying aberrant 3'-end formation, partial formation of cap 4, and overaccumulation in the cytoplasm; U₂₈ pseudouridylation was unaffected.

G.M.Z. and S.F. contributed equally to this work.

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