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Eukaryotic Cell, February 2004, p. 108-120, Vol. 3, No. 1
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.1.108-120.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

The Ras/Protein Kinase A Pathway Acts in Parallel with the Mob2/Cbk1 Pathway To Effect Cell Cycle Progression and Proper Bud Site Selection

Lisa Schneper, Alicia Krauss,{dagger} Ryan Miyamoto,{ddagger} Shirley Fang, and James R. Broach*

Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544

Received 26 June 2003/ Accepted 24 October 2003

In Saccharomyces cerevisiae, Ras proteins connect nutrient availability to cell growth through regulation of protein kinase A (PKA) activity. Ras proteins also have PKA-independent functions in mitosis and actin repolarization. We have found that mutations in MOB2 or CBK1 confer a slow-growth phenotype in a ras2{Delta} background. The slow-growth phenotype of mob2{Delta} ras2{Delta} cells results from a G1 delay that is accompanied by an increase in size, suggesting a G1/S role for Ras not previously described. In addition, mob2{Delta} strains have imprecise bud site selection, a defect exacerbated by deletion of RAS2. Mob2 and Cbk1 act to properly localize Ace2, a transcription factor that directs daughter cell-specific transcription of several genes. The growth and budding phenotypes of the double-deletion strains are Ace2 independent but are suppressed by overexpression of the PKA catalytic subunit, Tpk1. From these observations, we conclude that the PKA pathway and Mob2/Cbk1 act in parallel to determine bud site selection and promote cell cycle progression.

* Corresponding author. Mailing address: Department of Molecular Biology, Princeton University, Princeton, NJ 08544. Phone: (609) 258-5981. Fax: (609) 258-1975. E-mail: jbroach{at}molbio.princeton.edu.

{dagger} Present address: DuPont Crop Genetics, Newark, DE 19711.

{ddagger} Present address: University of Maryland, School of Medicine, Baltimore, MD 21210.

Eukaryotic Cell, February 2004, p. 108-120, Vol. 3, No. 1
1535-9778/04/$08.00+0     DOI: 10.1128/EC.3.1.108-120.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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