Eukaryotic Cell
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Eukaryotic Cell, August 2003, p. 821-825, Vol. 2, No. 4
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.4.821-825.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Specific Protein Targeting during Cell Differentiation: Polarized Localization of Fus1p during Mating Depends on Chs5p in Saccharomyces cerevisiae

Beatriz Santos{dagger} and Michael Snyder*

Molecular, Cellular, and Developmental Biology Department, Yale University, New Haven, Connecticut 06520-8103

Received 3 March 2003/ Accepted 8 May 2003

In budding yeast, chs5 mutants are defective in chitin synthesis and cell fusion during mating. Chs5p is a late-Golgi protein required for the polarized transport of the chitin synthase Chs3p to the membrane. Here we show that Chs5p is also essential for the polarized targeting of Fus1p, but not of other cell fusion proteins, to the membrane during mating.

* Corresponding author. Mailing address: MCDB Department, P.O. Box 208103, Yale University, New Haven, CT 06520-8103. Phone: (203) 432-6139. Fax: (203) 432-6161. E-mail: michael.snyder{at}yale.edu.

{dagger} Present address: Instituto Microbiología-Bioquímica, Departamento de Microbiología-Genética, CSIC/Universidad de Salamanca, 37007 Salamanca, Spain.

Eukaryotic Cell, August 2003, p. 821-825, Vol. 2, No. 4
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.4.821-825.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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