This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ramón, A. M.
Right arrow Articles by Fonzi, W. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramón, A. M.
Right arrow Articles by Fonzi, W. A.

 Previous Article  |  Next Article 

Eukaryotic Cell, August 2003, p. 718-728, Vol. 2, No. 4
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.4.718-728.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Diverged Binding Specificity of Rim101p, the Candida albicans Ortholog of PacC

Ana M. Ramón and William A. Fonzi*

Department of Microbiology and Immunology, Georgetown University, Washington, DC 20057-2197

Received 14 February 2003/ Accepted 29 April 2003

The biology of Candida albicans, including dimorphism and virulence, is significantly influenced by environmental pH. The response to ambient pH includes the pH-conditional expression of several genes, which is directly or indirectly regulated by Rim101p. Rim101p is homologous to PacC, a transcription factor that regulates pH-conditional gene expression in Aspergillus nidulans. PacC binds 5'-GCCARG-3' sequences upstream of pH-responsive genes and either activates or represses transcription. The absence of pacC consensus binding sites upstream of PHR1, a RIM101-dependent, alkaline pH-induced gene of C. albicans, suggested either that PHR1 is indirectly regulated by Rim101p or that the binding specificity of Rim101p is different. In vitro binding studies demonstrated that Rim101p strongly bound two regions upstream of PHR1 that were only weakly bound by PacC. Deletion analysis and site-specific mutagenesis demonstrated that both sites were functionally significant, mutation of either site reduced RIM101-dependent induction, and expression was abolished in the double mutant. Furthermore, oligonucleotides containing these sites conferred pH-conditional expression when inserted upstream of a reporter gene. The consensus sequence of these sites, 5'-CCAAGAAA-3', was identical to the binding recognition sequence identified by in vitro selection of Rim101p binding oligonucleotides from a random pool. The functional significance of this binding sequence was reinforced by its observed presence upstream of a number of newly identified pH-conditional genes. We conclude that Rim101p acts as a transcription factor and directly regulates pH-conditional gene expression but has a binding specificity different from that of PacC.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Georgetown University, 3900 Reservoir Rd., N.W., Washington, DC 20057-2197. Phone: (202) 687-1135. Fax: (202) 687-1800. E-mail: fonziw{at}georgetown.edu.

Eukaryotic Cell, August 2003, p. 718-728, Vol. 2, No. 4
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.4.718-728.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Strijbis, K., van Roermund, C. W. T., Hardy, G. P., van den Burg, J., Bloem, K., de Haan, J., van Vlies, N., Wanders, R. J. A., Vaz, F. M., Distel, B. (2009). Identification and characterization of a complete carnitine biosynthesis pathway in Candida albicans. FASEB J. 23: 2349-2359 [Abstract] [Full Text]  
  • Johnston, D. A., Eberle, K. E., Sturtevant, J. E., Palmer, G. E. (2009). Role for Endosomal and Vacuolar GTPases in Candida albicans Pathogenesis. Infect. Immun. 77: 2343-2355 [Abstract] [Full Text]  
  • Rolland, S., Bruel, C., Rascle, C., Girard, V., Billon-Grand, G., Poussereau, N. (2009). pH controls both transcription and post-translational processing of the protease BcACP1 in the phytopathogenic fungus Botrytis cinerea. Microbiology 155: 2097-2105 [Abstract] [Full Text]  
  • Chaffin, W. L. (2008). Candida albicans Cell Wall Proteins. Microbiol. Mol. Biol. Rev. 72: 495-544 [Abstract] [Full Text]  
  • Blanchin-Roland, S., Da Costa, G., Gaillardin, C. (2008). Ambient pH signalling in the yeast Yarrowia lipolytica involves YlRim23p/PalC, which interacts with Snf7p/Vps32p, but does not require the long C terminus of YlRim9p/PalI. Microbiology 154: 1668-1676 [Abstract] [Full Text]  
  • Strijbis, K., van Roermund, C. W. T., Visser, W. F., Mol, E. C., van den Burg, J., MacCallum, D. M., Odds, F. C., Paramonova, E., Krom, B. P., Distel, B. (2008). Carnitine-Dependent Transport of Acetyl Coenzyme A in Candida albicans Is Essential for Growth on Nonfermentable Carbon Sources and Contributes to Biofilm Formation. Eukaryot Cell 7: 610-618 [Abstract] [Full Text]  
  • Biswas, S., Van Dijck, P., Datta, A. (2007). Environmental Sensing and Signal Transduction Pathways Regulating Morphopathogenic Determinants of Candida albicans. Microbiol. Mol. Biol. Rev. 71: 348-376 [Abstract] [Full Text]  
  • Palmer, G. E., Kelly, M. N., Sturtevant, J. E. (2007). Autophagy in the pathogen Candida albicans. Microbiology 153: 51-58 [Abstract] [Full Text]  
  • Piekarska, K., Mol, E., van den Berg, M., Hardy, G., van den Burg, J., van Roermund, C., MacCallum, D., Odds, F., Distel, B. (2006). Peroxisomal Fatty Acid {beta}-Oxidation Is Not Essential for Virulence of Candida albicans. Eukaryot Cell 5: 1847-1856 [Abstract] [Full Text]  
  • Baek, Y.-U., Martin, S. J., Davis, D. A. (2006). Evidence for Novel pH-Dependent Regulation of Candida albicans Rim101, a Direct Transcriptional Repressor of the Cell Wall {beta}-Glycosidase Phr2.. Eukaryot Cell 5: 1550-1559 [Abstract] [Full Text]  
  • Villar, C. C., Kashleva, H., Mitchell, A. P., Dongari-Bagtzoglou, A. (2005). Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection. Infect. Immun. 73: 4588-4595 [Abstract] [Full Text]  
  • Barwell, K. J., Boysen, J. H., Xu, W., Mitchell, A. P. (2005). Relationship of DFG16 to the Rim101p pH Response Pathway in Saccharomyces cerevisiae and Candida albicans. Eukaryot Cell 4: 890-899 [Abstract] [Full Text]  
  • Noble, S. M., Johnson, A. D. (2005). Strains and Strategies for Large-Scale Gene Deletion Studies of the Diploid Human Fungal Pathogen Candida albicans. Eukaryot Cell 4: 298-309 [Abstract] [Full Text]  
  • Palmer, G. E., Johnson, K. J., Ghosh, S., Sturtevant, J. (2004). Mutant alleles of the essential 14-3-3 gene in Candida albicans distinguish between growth and filamentation. Microbiology 150: 1911-1924 [Abstract] [Full Text]  
  • Lotz, H., Sohn, K., Brunner, H., Muhlschlegel, F. A., Rupp, S. (2004). RBR1, a Novel pH-Regulated Cell Wall Gene of Candida albicans, Is Repressed by RIM101 and Activated by NRG1. Eukaryot Cell 3: 776-784 [Abstract] [Full Text]  
  • Staib, P., Binder, A., Kretschmar, M., Nichterlein, T., Schroppel, K., Morschhauser, J. (2004). Tec1p-Independent Activation of a Hypha-Associated Candida albicans Virulence Gene during Infection. Infect. Immun. 72: 2386-2389 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»