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Eukaryotic Cell, June 2003, p. 569-577, Vol. 2, No. 3
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.3.569-577.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Dyskinetoplastic Trypanosoma brucei Contains Functional Editing Complexes

Gonzalo J. Domingo,1,2 Setareh S. Palazzo,1,2 Bingbing Wang,1,2 Brian Pannicucci,1 Reza Salavati,1,2 and Kenneth D. Stuart1,2*

Seattle Biomedical Research Institute, Seattle, Washington 98109,1 Department of Pathobiology, University of Washington, Seattle, Washington 981952

Received 7 October 2002/ Accepted 15 January 2003

Mitochondrial pre-mRNAs undergo posttranscriptional RNA editing as directed by small guide RNAs (gRNAs) to produce functional mRNAs in kinetoplastid protozoa. The pre-mRNAs and gRNAs are encoded in the maxicircle and minicircle components, respectively, of the kinetoplastid mitochondrial DNA (kDNA), and editing is catalyzed by a multienzyme protein complex. Trypanosoma evansi AnTat3/3, which lacks maxicircles but retains a single class of minicircles, and a dyskinetoplastic mutant of Trypanosoma brucei EATRO164, which is devoid of kDNA, were both shown to retain genes and proteins for the editing complex. The proteins are present in complexes that immunoprecipitate and sediment indistinguishably from wild-type complexes. The complexes catalyze precleaved insertion and deletion editing as well as full-round deletion editing in vitro. Thus, mutants which lack the natural substrates for RNA editing and all or most gRNAs retain editing complexes that contain the four primary catalytic activities of editing and function in editing, at least in vitro. Therefore neither pre-mRNA nor gRNA is required to form functional RNA-editing complexes.

* Corresponding author. Mailing address: Seattle Biomedical Research Institute, 4 Nickerson St., Seattle, WA 98109. Phone: (206) 284-8846 ext. 316. Fax: (206) 284-0313. E-mail: kstuart{at}u.washington.edu.

Eukaryotic Cell, June 2003, p. 569-577, Vol. 2, No. 3
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.3.569-577.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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