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Eukaryotic Cell, June 2003, p. 465-473, Vol. 2, No. 3
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.3.465-473.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

A Gene Expressed during Sexual and Asexual Sporulation in Phytophthora infestans Is a Member of the Puf Family of Translational Regulators

Cristina Cvitanich and Howard S. Judelson*

Department of Plant Pathology, University of California, Riverside, California 92521

Received 31 July 2002/ Accepted 20 February 2003

A gene from Phytophthora infestans that was previously identified as being induced during the development of sexual spores was also found to be active during asexual sporulation. The gene, M90, was expressed as a 3.1-kb primary transcript containing two introns and was predicted to encode a member of the Puf family of translational regulators. The protein showed up to 51% amino acid identity to other Puf proteins within its 353-amino-acid RNA-binding domain. Little similarity extended beyond this region, as noted for other members of the family. Expression of M90 was measured by using RNA blots and transformants of P. infestans expressing a fusion between the M90 promoter and the ß-glucuronidase (GUS) gene. A 1.3-kb promoter fragment conferred the normal M90 pattern of expression to the GUS reporter in transformants. In matings, expression was first detected in male and female gametangial initials and persisted in mature oospores. Expression was also observed in hyphal tips just prior to asexual sporulation, in sporangiophores, in mature sporangia, and in zoospores. The signal quickly disappeared once spores made the transition to hyphae after germination. Nutrient limitation did not induce the gene. Potential roles for a translational regulator during both sexual development and asexual sporulation are discussed.

* Corresponding author. Mailing address: Department of Plant Pathology, University of California, Riverside, CA 92521. Phone: (909) 787-4199. Fax: (909) 787-4294. E-mail: Howard.Judelson{at}ucr.edu.

Eukaryotic Cell, June 2003, p. 465-473, Vol. 2, No. 3
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.3.465-473.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ah-Fong, A. M. V., Xiang, Q., Judelson, H. S. (2007). Architecture of the Sporulation-Specific Cdc14 Promoter from the Oomycete Phytophthora infestans. Eukaryot Cell 6: 2222-2230 [Abstract] [Full Text]  
  • Tani, S., Judelson, H. (2006). Activation of Zoosporogenesis-Specific Genes in Phytophthora infestans Involves a 7-Nucleotide Promoter Motif and Cold-Induced Membrane Rigidity.. Eukaryot Cell 5: 745-752 [Abstract] [Full Text]  
  • Kim, K. S., Judelson, H. S. (2003). Sporangium-Specific Gene Expression in the Oomycete Phytopathogen Phytophthora infestans. Eukaryot Cell 2: 1376-1385 [Abstract] [Full Text]  


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