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 Previous Article

Eukaryotic Cell, April 2003, p. 362-379, Vol. 2, No. 2
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.2.362-379.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Map of the Chlamydomonas reinhardtii Nuclear Genome

Pushpa Kathir, Matthew LaVoie, William J. Brazelton, Nancy A. Haas, Paul A. Lefebvre, and Carolyn D. Silflow*

Department of Genetics, Cell Biology, and Development and Department of Plant Biology, University of Minnesota, St. Paul, Minnesota

Received 12 August 2002/ Accepted 10 December 2002

We have prepared a molecular map of the Chlamydomonas reinhardtii genome anchored to the genetic map. The map consists of 264 markers, including sequence-tagged sites (STS), scored by use of PCR and agarose gel electrophoresis, and restriction fragment length polymorphism markers, scored by use of Southern blot hybridization. All molecular markers tested map to one of the 17 known linkage groups of C. reinhardtii. The map covers approximately 1,000 centimorgans (cM). Any position on the C. reinhardtii genetic map is, on average, within 2 cM of a mapped molecular marker. This molecular map, in combination with the ongoing mapping of bacterial artificial chromosome (BAC) clones and the forthcoming sequence of the C. reinhardtii nuclear genome, should greatly facilitate isolation of genes of interest by using positional cloning methods. In addition, the presence of easily assayed STS markers on each arm of each linkage group should be very useful in mapping new mutations in preparation for positional cloning.

* Corresponding author. Mailing address: Department of Plant Biology, 250 Bioscience Center, University of Minnesota, 1445 Gortner Ave., St. Paul, MN 55108. Phone: (612) 624-0729. Fax: (612) 625-1738. E-mail: carolyn{at}biosci.cbs.umn.edu.

Eukaryotic Cell, April 2003, p. 362-379, Vol. 2, No. 2
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.2.362-379.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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