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Eukaryotic Cell, April 2003, p. 222-230, Vol. 2, No. 2
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.2.222-230.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Exportin 1 Mediates Nuclear Export of the Kinetoplastid Spliced Leader RNA

Gusti M. Zeiner, Nancy R. Sturm, and David A. Campbell*

Department of Microbiology, Immunology & Molecular Genetics, University of California at Los Angeles, Los Angeles, California 90095-1489

Received 6 December 2002/ Accepted 28 January 2003

The kinetoplastid protozoan spliced leader (SL) RNA is the common substrate pre-mRNA utilized in all trans-splicing reactions. Here we show by fluorescence in situ hybridization that the SL RNA is present in the cytoplasm of Leishmania tarentolae and Trypanosoma brucei. Treatment with the karyopherin-specific inhibitor leptomycin B was toxic to T. brucei and eliminated the cytoplasmic SL RNA, suggesting that cytoplasmic SL RNA was dependent on the nuclear exporter exportin 1 (XPO1). Ectopic expression of xpo1 with a C506S mutation in T. brucei conferred resistance to leptomycin B. A reduction in SL RNA 3' extension removal and 5' methylation of nucleotide U4 was observed in wild-type T. brucei treated with leptomycin B, suggesting that the cytoplasmic stage is necessary for SL RNA biogenesis. This study demonstrates spatial and mechanistic similarities between the posttranscriptional trafficking of the kinetoplastid protozoan SL RNA and the metazoan cis-spliceosomal small nuclear RNAs.

* Corresponding author. Mailing address: Department of Microbiology, Immunology & Molecular Genetics, University of California at Los Angeles, 609 Charles E. Young Dr. East, Los Angeles, CA 90095-1489. Phone: (310) 206-5556. Fax: (310) 206-5231. E-mail: dc{at}ucla.edu.

Eukaryotic Cell, April 2003, p. 222-230, Vol. 2, No. 2
1535-9778/03/$08.00+0     DOI: 10.1128/EC.2.2.222-230.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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