This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow E-mail this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhang, M.
Right arrow Articles by Erdman, S. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, M.
Right arrow Articles by Erdman, S. E.

 Previous Article  |  Next Article 

Eukaryotic Cell, October 2002, p. 811-822, Vol. 1, No. 5
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.5.811-822.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Maintenance of Mating Cell Integrity Requires the Adhesin Fig2p

Mingliang Zhang, Daniel Bennett, and Scott E. Erdman*

Department of Biology, Syracuse University, Syracuse, New York 13244-1220

Received 11 March 2002/ Accepted 22 July 2002

Fungal adhesins represent a large family of serine/threonine-rich secreted glycoproteins. Adhesins have been shown to play roles in heterotypic and homotypic cell-cell adhesion processes, morphogenetic pathways and invasive/pseudohyphal growth, frequently in response to differentiation cues. Here we address the role of the Saccharomyces cerevisiae mating-specific adhesin Fig2p. Cells lacking FIG2 possess a variety of mating defects that relate to processes involving the cell wall, including morphogenetic defects, cell fusion defects, and alterations in agglutination activities. We found that mating-specific morphogenetic defects caused by the absence of FIG2 are suppressible by increased external osmolarity and that, during mating, fig2{Delta} cells display reduced viability relative to wild-type cells. These defects result from alterations in signaling activated by the mating and cell integrity pathways. Finally, we show that fig2{Delta} zygotes also have defects in zygotic spindle positioning that are osmoremedial, whereas the requirements for FIG2 in normal cell-cell agglutination and cell fusion during mating are insensitive to changes in the extracellular osmotic environment. We conclude that FIG2 performs distinct functions in the mating cell wall that are separable with respect to their ability to be suppressed by changes in external osmolarity and that a fundamental role of FIG2 in mating cells is the maintenance of cell integrity.

* Corresponding author. Mailing address: Department of Biology, Syracuse University, Syracuse, NY 13244-1220. Phone: (315) 443-3748. Fax: (315) 443-2012. E-mail: seerdm{at}syr.edu.

Eukaryotic Cell, October 2002, p. 811-822, Vol. 1, No. 5
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.5.811-822.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Birkaya, B., Maddi, A., Joshi, J., Free, S. J., Cullen, P. J. (2009). Role of the Cell Wall Integrity and Filamentous Growth Mitogen-Activated Protein Kinase Pathways in Cell Wall Remodeling during Filamentous Growth. Eukaryot Cell 8: 1118-1133 [Abstract] [Full Text]  
  • Huang, G., Dougherty, S. D., Erdman, S. E. (2009). Conserved WCPL and CX4C Domains Mediate Several Mating Adhesin Interactions in Saccharomyces cerevisiae. Genetics 182: 173-189 [Abstract] [Full Text]  
  • Paterson, J. M., Ydenberg, C. A., Rose, M. D. (2008). Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating. JCB 181: 697-709 [Abstract] [Full Text]  
  • Dranginis, A. M., Rauceo, J. M., Coronado, J. E., Lipke, P. N. (2007). A Biochemical Guide to Yeast Adhesins: Glycoproteins for Social and Antisocial Occasions. Microbiol. Mol. Biol. Rev. 71: 282-294 [Abstract] [Full Text]  
  • Friesen, H., Humphries, C., Ho, Y., Schub, O., Colwill, K., Andrews, B. (2006). Characterization of the Yeast Amphiphysins Rvs161p and Rvs167p Reveals Roles for the Rvs Heterodimer In Vivo. Mol. Biol. Cell 17: 1306-1321 [Abstract] [Full Text]  
  • Barale, S., McCusker, D., Arkowitz, R. A. (2004). The Exchange Factor Cdc24 Is Required for Cell Fusion during Yeast Mating. Eukaryot Cell 3: 1049-1061 [Abstract] [Full Text]  
  • Huang, G., Zhang, M., Erdman, S. E. (2003). Posttranslational Modifications Required for Cell Surface Localization and Function of the Fungal Adhesin Aga1p. Eukaryot Cell 2: 1099-1114 [Abstract] [Full Text]  
  • Jue, C. K., Lipke, P. N. (2002). Role of Fig2p in Agglutination in Saccharomyces cerevisiae. Eukaryot Cell 1: 843-845 [Abstract] [Full Text]  


Copyright © 2010 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»