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Eukaryotic Cell, October 2002, p. 673-686, Vol. 1, No. 5
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.5.673-686.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Dual Role of the Saccharomyces cerevisiae TEA/ATTS Family Transcription Factor Tec1p in Regulation of Gene Expression and Cellular Development

Tim Köhler, Stefanie Wesche,{dagger} Naimeh Taheri, Gerhard H. Braus, and Hans-Ulrich Mösch*

Institute for Microbiology and Genetics, Georg-August University, D-37077 Göttingen Germany

Received 9 July 2002/ Accepted 31 July 2002

In Saccharomyces cerevisiae, the transcription factors Tec1p and Ste12p are required for haploid invasive and diploid pseudohyphal growth. Tec1p and Ste12p have been postulated to regulate these developmental processes primarily by cooperative binding to filamentous and invasion-responsive elements (FREs), which are combined enhancer elements that consist of a Tec1p-binding site (TCS) and an Ste12p-binding site (PRE). They are present in the promoter regions of target genes, e.g., FLO11. Here, we show that Tec1p efficiently activates target gene expression and cellular development in the absence of Ste12p. We further demonstrate that TCS elements alone are sufficient to mediate Tec1p-driven gene expression by a mechanism termed TCS control that is operative even when Ste12p is absent. Mutational analysis of TEC1 revealed that TCS control, FLO11 expression, and haploid invasive growth require the C terminus of Tec1p. In contrast, the Ste12p-dependent FRE control mechanism is sufficiently executed by the N-terminal portion of Tec1p, which contains the TEA/ATTS DNA-binding domain. Our study suggests that regulation of haploid invasive and diploid pseudohyphal growth by Ste12p and Tec1p is not only executed by combinatorial control but involves additional control mechanisms in which Ste12p activates TEC1 expression via clustered PREs and where Tec1p regulates expression of target genes, e.g., FLO11, by TCS control.

* Corresponding author. Mailing address: Institute for Microbiology and Genetics, Georg-August University, Grisebachstr. 8, D-37077 Göttingen, Germany. Phone: (49) 551 39 37 77. Fax: (49) 551 39 38 20. E-mail: hmoesch{at}gwdg.de.

{dagger} Present address: University of Heidelberg, Heidelberg, Germany

Eukaryotic Cell, October 2002, p. 673-686, Vol. 1, No. 5
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.5.673-686.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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Copyright © 2002 by the American Society for Microbiology.