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Eukaryotic Cell, August 2002, p. 568-582, Vol. 1, No. 4
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.4.568-582.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Sgt1p Contributes to Cyclic AMP Pathway Activity and Physically Interacts with the Adenylyl Cyclase Cyr1p/Cdc35p in Budding Yeast

Caroline Dubacq,1 Raphaël Guerois,2 Régis Courbeyrette,1 Katsumi Kitagawa,3 and Carl Mann1*

Service de Biochimie et de Génétique Moléculaire ,1 Service de Biophysique des Fonctions Membranaires, CEA/Saclay, F-91191 Gif-sur-Yvette, France,2 Department of Molecular Pharmacology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105-27943

Received 3 December 2001/ Accepted 29 April 2002

Sgt1p is a highly conserved eucaryotic protein that is required for both SCF (Skp1p/Cdc53p-Cullin-F-box)-mediated ubiquitination and kinetochore function in yeast. We show here that Sgt1p is also involved in the cyclic AMP (cAMP) pathway in Saccharomyces cerevisiae. SGT1 is an allele-specific suppressor of cdc35-1, a thermosensitive mutation in the leucine-rich repeat domain of the adenylyl cyclase Cyr1p/Cdc35p. We demonstrate that Sgt1p and Cyr1p/Cdc35p physically interact and that the activity of the cAMP pathway is affected in an sgt1 conditional mutant. Sequence analysis suggests that Sgt1p has features of a cochaperone. Thus, Sgt1p is a novel activator of adenylyl cyclase in S. cerevisiae and may function in the assembly or the conformational activation of specific multiprotein complexes.

* Corresponding author. Mailing address: SBGM, CEA/Saclay, F-91191 Gif-sur-Yvette, France. Phone: 33-1-69 08 34 32. Fax: 33-1-69 08 47 12. E-mail: mann{at}jonas.saclay.cea.fr.

Eukaryotic Cell, August 2002, p. 568-582, Vol. 1, No. 4
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.4.568-582.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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