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Eukaryotic Cell, April 2002, p. 241-248, Vol. 1, No. 2
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.2.241-248.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Aspergillus nidulans swoF Encodes an N-Myristoyl Transferase

Brian D. Shaw,1 Cory Momany,2 and Michelle Momany1*

Department of Botany,1 Department of Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, Georgia 306022

Received 6 December 2001/ Accepted 15 January 2002

Polar growth is a fundamental process in filamentous fungi and is necessary for disease initiation in many pathogenic systems. Previously, swoF was identified in Aspergillus nidulans as a single-locus, temperature-sensitive (ts) mutant aberrant in both polarity establishment and polarity maintenance. The swoF gene was cloned by complementation of the ts phenotype and sequenced. The derived protein sequence had high identity with N-myristoyl transferases (NMTs) found in fungi, plants, and animals. In addition, wild-type growth at restrictive temperature was partially restored by the addition of myristic acid to the growth medium. Sequencing revealed that the mutation in swoF changes the conserved aspartic acid 369 to a tyrosine. The predicted A. nidulans SwoF protein, SwoFp, was homology modeled based on crystal structures of NMTs from Saccharomyces cerevisiae and Candida albicans. The D369Y swoF mutation is on the opposite face of the protein, distal to the myristoyl coenzyme A and peptide substrate binding sites. In wild-type NMTs, D369 appears to stabilize a structural ß-strand bend through two hydrogen bonds and an ionic interaction. These stabilizing bonds are abolished in the D369Y mutant. We hypothesize that a substrate of SwoFp must be myristoylated for proper polarity establishment and maintenance. The mutation prevents the proper function of SwoFp at restrictive temperature and thus blocks polar growth.

* Corresponding author. Mailing address: Department of Botany, University of Georgia, Athens, GA 30602. Phone: (706) 542-2014. Fax: (706) 542-1805. E-mail: momany{at}botany.uga.edu.

Eukaryotic Cell, April 2002, p. 241-248, Vol. 1, No. 2
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.2.241-248.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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