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Eukaryotic Cell, April 2002, p. 229-240, Vol. 1, No. 2
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.2.229-240.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Nnf1p, Dsn1p, Mtw1p, and Nsl1p: a New Group of Proteins Important for Chromosome Segregation in Saccharomyces cerevisiae

Ghia M. Euskirchen*

Department of Biological Sciences, Columbia University, New York, New York 10027

Received 22 August 2001/ Accepted 10 January 2002

Previously, antibodies were raised against a nuclear envelope-enriched fraction of yeast, and the essential gene NNF1 was cloned by reverse genetics. Here it is shown that the conditional nnf1-17 mutant has decreased stability of a minichromosome in addition to mitotic spindle defects. I have identified the novel essential genes DSN1, DSN3, and NSL1 through genetic interactions with nnf1-17. Dsn3p was found to be equivalent to the kinetochore protein Mtw1p. By indirect immunofluorescence, all four proteins, Nnf1p, Mtw1p, Dsn1p, and Nsl1p, colocalize and are found in the region of the spindle poles. Based on the colocalization of these four proteins, the minichromosome instability and the spindle defects seen in nnf1 mutants, I propose that Nnf1p is part of a new group of proteins necessary for chromosome segregation.

* Present address: Yale University, Department of Molecular, Cellular, and Developmental Biology, KBT Room 918, 266 Whitney Ave., New Haven, CT 06511. Phone: (203) 432-3510. Fax: (203) 432-6161. E-mail: ghia.euskirchen{at}yale.edu.

Eukaryotic Cell, April 2002, p. 229-240, Vol. 1, No. 2
1535-9778/02/$04.00+0     DOI: 10.1128/EC.1.2.229-240.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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