Strain and relevant genotype Sp act (mU/mg protein) of β-galactosidase Glucose Glycerol Ethanol Acetate YNB YP YNB YP YNB YP YNB YP RJM007 YlFBP1 36 ± 5 14 ± 4 47 ± 4 12 ± 1 83 ± 6 36 ± 3 70 ± 5 23 ± 5 RJM008 Ylfbp1::URA3 97 ± 13 33 ± 2 94 ± 15 23 ± 5 297 ± 15 182 ± 6 372 ± 13 189 ± 10

^a Y. lipolytica strains RJM007 and RJM008, wild type and Ylfbp1::URA3, respectively, bearing a fusion of the YlFBP1 promoter to the E. coli lacZ integrated into the chromosomal YlLEU2 locus (see Materials and Methods) were cultured in minimal or rich medium with the indicated carbon sources, and β-galactosidase was assayed as described in Materials and Methods. Results are the mean values ± the standard errors of the means of the results from four independent cultures.